high-throughput sequencing system

Contact：Xianbin Su
Tel：34204150

MORE <p><strong>Model：</strong>illumina HiSeq 2500</p> <p> </p> <p><strong>Specifications：</strong><br /> 1、 Rapid mode (2 lane flow cell) or High Output mode(8 lane flow cell);<br /> 2、Two flow cell can be operated at the same time;<br /> 3、Rapid mode: up to 300 million read count per output, support 1×36，2×50，2×100，2×150，2×250 bp read length; High Output mode: up to 3 billion single read or 6 billion paired-end reads, support 1×36，2×50，2×100，2×125 bp  read length;<br /> 4、Quality score: Q30>80%；<br /> 5、It can generate up to 1 Tb data within dual flow cell run time of 6 days.</p> <p><br /> <strong>Applications：</strong><br /> 1、Whole genome sequencing；<br /> 2、 Complete transcriptome sequencing;<br /> 3、 Whole exome sequencing;<br /> 4、 Target sequencing; <br /> 5、 De novo sequencing; <br /> 6、 Single cell sequencing<br />  </p>

Micromanipulation system

Contact: Man Zhang
Tel:34206535

MORE <p><strong>Model：</strong>XenoWorks TM</p> <p><br /> <strong>Specifications：</strong><br />     1、The micromanipulation moves in six directions with a maximum displacement of 25 mm. The operation mode can be customized. The maximum pressure injection can be up to 5600 hpa, with high sensitivity LED display.<br />     2、The objective has the function of DIC;<br />     3、Analyzer;<br />     4、Six hole revolving nosepiece;<br />     5、Equipped with 2 million pixel monochrome CCD, software and special interface.</p> <p> </p> <p><strong>Applications：</strong><br />  A method of cell or early embryo manipulation by using a micromanipulator to control the movement of microinjection needle in the field of view of a microscope under a high power inverted microscope. It can be used for precise microinjection, gene cloning, patch clamp injection, nuclear transfer and embryo transfer.<br />  </p>

Automatic patchclamp

Contact: Man Zhang
Tel:34206535

MORE <p><strong>Model：</strong>Patchliner Octo</p> <p> </p> <p><strong>Specifications：</strong><br />    1、High-throughput: number of channel is up to 8, daily obtained data is up to 250-500 data points;<br />    2、Whole cell recording, single channel current recording;<br />    3、Current clamp mode and voltage clamp mode;<br />    4、Whole cell patch recording seal resistance is bigger than 1G ohm, series current compensation, success rate is bigger than 60 percent;<br />    5、Automatic data analysis;<br />    6、Internal and external cellular perfusion;<br />    7、Temperature control is available.<br />   <br /> <strong>Applications：</strong><br />    It is a highly automatic and integrated platform of the latest for measuring ion channel information of cell membrane.<br />    1、It is used for testing the current change of ion channel on animal, plant cells or protein.<br />    2、High-throughput drug screening;<br />    3、To study the effects of various inhibitors and blockers on the target protein and the mechanisms of intracellular regulation.<br />  </p>

Atomic force microscope

Contact：Man Zhang
Tel：34206535

MORE <p><strong>Model：</strong>Bruker Dimension Icon/Multimode 8<br /> <br /> <strong>Specifications：</strong><br />     1、Contact AFM;<br />     2、Same mode as “Tapping Mode”;<br />     3、Scanning mode of automatic parameter optimization: the system can automatically optimize the parameter, ensure the stability of environmental testing in the liquid environment, it is conductive to the study of living cells and other biological samples;<br />     4、Quantitative mechanical test mode: the resolution reach highest to atomic level;<br />     5、Force curve and force spectrum: the maximum point density is no less than 256×256;<br />     6、Nano etching and nano manipulation mode: the sample surface can be automatically etched with nano precision  according to the setting parameter, or the single molecules on the substrate surface can be manipulated with nano precision;<br />     7、The probe microcantilever elastic constant can be calibrated by thermal vibration, and the calibration range is ≥2MHz;<br />     8、Simultaneous data acquisition mode of 8 channels or above<br />    <br /> <strong>Applications：</strong><br />    1、Characterization of morphology and structure of biomaterials, such as DNA, cell, etc.;<br />    2、Dynamic process of living cells;<br />    3、Biomechanical study(single molecule force spectrum, protein unfolding , etc.);<br />    4、Analysis and manipulation of  biocompatible materials such as nano gold;<br />    5、Describe the magnetic distribution of magnetic molecules or particles;  <br />    6、Electrical distributions of liquid crystal materials and external field influence   <br />  </p>

Inverted fluorescence phase contrast microscope

Contact：Man Zhang
Tel：34206535

MORE <p><strong>Model：</strong>Nikon Ti-U<br /> <br /> <strong>Specifications：</strong><br />     1、Layered structure design;<br />     2、Fluorescent lighting system: long life metal short arc lamp lighting;<br />     3、Objective: 4、10、20、40、60 times flat field half apochromatic objective;<br />     4、Two 10 times eye lens: broad views, mould proof type;<br />     5、Automatic focusing system;<br />     6、UV, blue, green fluorescent filter blocks;<br />     7、High resolution refrigeration imaging system and color imaging system配有高分辨率制冷成像系统和彩色成像系统；<br />     8、Functions such as bright field, phase contrast, fluorescence,etc. <br /> <br /> <strong>Applications：</strong><br />    It is mainly used in living cell observations and detection of fluorescence changes of fluorescent dye labeling in living cells.</p>

Ultra high-performance liquid chromatography -quadrupole-time of flight mass spectrometry

Contact：Chen, Ke
Tel：34208616

MORE <p><strong>Model：</strong> Agilent 6560 UPLC-QTOF-MS</p> <p><br /> <strong>Specifications：</strong><br /> 1、Flow rate range: 0.1-0.4 ml/min<br /> 2、Pressure range: 0-15000 psi<br /> 3、Column temperature range: 5-65 ℃<br /> 4、Mass-to-charge ratio（m/z）:100-4000 Da <br /> 5、Quality measurement precision: < 5ppm</p> <p><br /> <strong>Applications：</strong><br /> 1、Metabonomics: metabolite analysis of biological samples such as blood, urine, and cell;<br /> 2、Accurate molecular weight determination of organic compounds;<br /> 3、Qualitative and quantitative analysis of organic compounds;<br /> 4、MS/MS mode can be used for structural resolutions of target compound;<br /> 5、Analysis of Chinese traditional medicine and food</p>

protein purification system

Contact：Qi, Huan
Tel：34207041

MORE <p><strong>Model：</strong>Akta Purifier 10</p> <p><br /> <strong>Specifications：</strong><br /> 1. Separation of  traditional Chinese medicine by medium pressure column chromatography;<br /> 2. Separation and preparation of Chinese medicine monomer;<br /> 3. Separation and purification of drug protein;<br /> 4. Study on the refolding of drug protein after prokaryotic expression;<br /> 5. Explore the purification process and establish the method.</p> <p><br /> <strong>Applications：</strong><br />       The system pressure can reach up to 3650psi, and the buffer solution is automatically configured. Only high concentration buffer solution needs to be prepared, and the pH, water, acid and 2m salt solution do not need to be manually adjusted. The system will automatically prepare the required pH buffer solution for purification. Other automatic functions include automatic solution selection and large component switching, and detection functions include multi wavelength UV/visible light, conductivity, pH, temperature, etc.</p>

Gel permeation chromatograph

Contact：Li, Xiaolin
Tel：34204561

MORE <p><strong>Model：</strong> Agilent  1260</p> <p> </p> <p><strong>Specifications：</strong><br />    1、Maximum flow rate of liquid phase pump 10ml/min;<br />    2、Temperature control range of chromatographic column is up to 80 ℃;<br />    3、Temperature upper limit of differential refraction detector is 55 ℃<br /> <strong> </strong></p> <p><strong>Applications：</strong><br />     This is a professional equipment for macromolecular research in the field of chemistry, biology and medicine.<br />     1、 It can measure the average molecular weight, molecular weight distribution and the width of molecular weight distribution;<br />     2、Analyze the homopolymer and impurity in the copolymer, and determine the composition distribution and molecular weight distribution of the copolymer;<br />     3、A powerful tool for analyzing  small molecules.</p>

Preparative liquid chromatograph

Contact：Li, Xiaolin
Tel：34204561

MORE <p><strong>Model：</strong> Agilent  1260</p> <p><br /> <strong>Specifications：</strong><br />    1、Working principle of the pump: the low-pressure four-way mixing pump integrates the high-performance degassing unit; <br />    2、Flow rate range: 0.05 - 5 mL/min at 600 bar; 5 - 10 mL/min at 200 bar;<br />    3、Maximum pressure: 600 bar;<br />    4、Injection principle: equipped with automatic injector;<br />    5、Injection range: 0.1 - 100 μL;   6、Detector data acquisition rate: UV detector 80 Hz；fluorescent detector 74 Hz；evaporative light scattering detector 60 Hz。</p> <p><br /> <strong>   Applications：</strong><br />    Preparative liquid chromatograph is a common equipment for processing multiple components complex systems in the field of separation and purification. The equipment can increase sample loading volumes through increasing the diameter of chromatographic column without decreasing the column efficiency, thus to separate, purify and  enrich the complex system of components, high value-added products and mixtures difficult to purify by conventional means.</p>

High performance liquid chromatograph

Contact：Li, Xiaolin
Tel：34206535

MORE <p><strong>Model：</strong> Shimadzu LC-20A</p> <p><br /> <strong>Specifications： </strong><br />    Binary high pressure gradient pump:<br />    1、Flow range: 0.0001-20.000ml/min;<br />    2、Flow precision: RSD≤0.1%;<br />    3、Pressure range: 0~40Mpa;<br />    4、Infusion mode: parallel double column plunger; automatic injector;<br />    5、Injection speed: 1 injection/10 second;<br />    6、Injection precision: RSD ≤ 0.3% ;<br />    7、Needle cleaning：automatic function;<br />    8、Temperature range: 4-40,dehumidification;<br />    9、Wavelength detection range: 190～800nm </p> <p><br /> <strong>Applications：</strong><br /> The equipment is mainly applied in proteomics, metabonomics and glycomics. It can separate amino acids, peptides, proteins, nucleic acids, biogenic amines, etc. It can also separate and detect substances of various molecular weight ranges such as thermal instability and non volatile, dissociated, and non dissociated and components in complex systems.</p>

Biochip scanner

Contact：Qi, Huan
Tel：34207041

MORE <p><strong>Model：</strong>Genepix4000A<br />     <br /> <strong>Specifications：</strong><br />     1、Numerical aperture >0.7，high signal to noise ratio;<br />     2、Focus adjustable>±200µm;<br />     3、Scanning range 22mm×70mm;<br />     4、Excitation wavelength: 488 nm, 532 nm, 594 nm, 635 nm；<br />     5、High sensitivity <0.1fluorescent molecule/µm2<br />     6、Laser intensity and continuously adjustable PMT;<br />     7、Dynamic range:104<br />     8、Scanning speed: 30sec/cm2<br />     9、Repeatability: 95%~98%;<br />     10、Four optional resolutions: 5、10、20、40µm<br />          <br /> <strong>Applications：</strong><br />     1、High, mid, low density biochip dual color or monochromatic fluorescence detection analysis;<br />     2、DNA methylation test and gene expression profile analysis;<br />     3、Clinical test, such as bacterial identification and drug resistance test, etc.</p>

SmartArrayer48 microarray spotter

Contact：Qi, Huan
Tel：34207041

MORE <p><strong>Model：</strong>Smart Arrayer48<br /> <br /> <strong>Specifications：</strong><br />   1、contact spotter and non contact spray sample two-in-one  biochip preparation system;<br />   2、48 samples at a time;<br />   3、skip point fucntion;<br />   4、make up the missing points;<br />   5、set up sampling quantity , loading position, loading orders at will;<br />   6、single cycle time<110s;<br />   7、contact minimum sample intake: 0.3µL，noncontact minimum sample intake: 3µL；<br />   8、contact minimum sampling quantity: 1nl，non contact minimum sampling quantity: 15nl<br />    </p> <p><strong>Applications：</strong><br />   1、Spot or spray gene/protein chips;<br />   2、non contact spray is suitable for multiple biological samples such as nucleic acid/protein/cell fragments, and can be carried out on the glass slides, films and 96 well plates.</p>

The ForteBio Octet Red96 Analyzer

Contact：Qi, Huan
Tel：34207041

MORE <p><strong>Model：</strong>Fortebio  Octet RED96</p> <p><br /> <strong>Specifications：</strong><br />      1、parallel analysis sample: 1-8;<br />      2、sample volume: 180~220μl;<br />      3、refractive index range：not affected by the refractive index of biological samples;<br />      4、sample temperature control: room temperature -40 ℃;<br />      5、range of binding constant: 101-107M-1s-1;<br />      6、dissociation constant range: 10-6—10-1s-1;<br />      7、equilibrium affinity:103-1012M-1;<br />      8、concentration range: 0.5μ g/ml-2000μg/ml;<br />      9、quantitative analysis time: 8 sample <2min，96 sample <30min </p> <p><br /> <strong>Applications:</strong><br />  Octet red96 system, on the one hand, can be used to measure the concentration of proteins and other biomolecules rapidly, and it is also an ideal choice for the measurement of affinity and kinetics, the study of protein-protein interaction and protein-small molecule interaction. It can be widely used in antibody and protein concentration measurement, quality analysis, antibody screening, cell line optimization, antigen epitope identification, antibody pairing, screening and analysis of small molecules and molecular fragments, research on the mechanism of intercellular signal transduction and detection of infectious diseases.</p> <p><br />  </p>

Liquid chromatograph-Orbitrap Fusion Tribrid一Mass Spectrometry

技术联系人：Ku, Xin
联系电话：34204138

MORE <p><strong>Model：</strong>Orbitrap Fusion </p> <p><br /> <strong>Specifications：</strong><br /> （1） Segmented Quadrupole Mass Filter for precursor ion selection with variable precursor isolation width from 0.4 u  to 1,200 u;<br /> （2） Ultra high-field Orbitrap analyzer for ultra high esolution OTMS >450,000, and up to 15Hz scanning rate, the fastest Orbitrap acquisition rates ; <br /> （3）  MS/MS Electrospray Ionization (ESI) Ion Trap sensitivity: femtograms; MS Scan Power: MSn, for n = 1 through 10; Collision-induced dissociation (CID), Higher-energy collisional dissociation (HCD) and optional electron-transfer dissociation (ETD) </p> <p> </p> <p><strong>Applications：</strong> The core of Orbitrap fusion LC-MS is a kind of three different quality analyzer structures, which can work together to improve the analysis performance to a new level. The system integrates quadrupole, orbit trap and linear ion trap, which can provide unprecedented depth for complex biological sample analysis. Precursor fragmentation can take place  in the ion-routing multipole (HCD), in the ion trap (CID, ETD) or both (EThcD) with fragment detection in the linear ion trap or Orbitrap mass analyzers at any stage of MSn analysis, so as to obtain new level of structural information from metabolites, polysaccharides, post-translational modifications and sequence polymorphism.</p>

Gas chromatography time of flight mass spectrometry

Contact：Wang, Xiaoyan
Tel：34207343

MORE <p><strong>Model：</strong>Pegasus IV GC/TOFMS</p> <p> </p> <p><strong>Specifications：</strong><br />    1、Range：1~1,500(m/z)    <br />    2、Sensitivity：5pg hexachlorobenzene (S/N>10:1)   <br />    3、Resolution：7,000(FWHM)<br />    4、Quantitative dynamic linear range：104~108<br />    </p> <p><strong>Applications：</strong><br />     1、Analysis of drug metabolite, qualitative and quantitative analysis of  low boiling point mixture;<br />     2、High resolution mass spectrometry detection of small molecular weight compounds.<br />  </p>