Raindrop digital PCR system

Contact：Su, Xianbin
Tel：34204150

MORE <p><strong>Model： RainDance RainDrop</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1、Achieve more than 1 million independent PCR reactions for a single sample at a time;</p> <p>2、It can detect 1/250,000 rare mutations;</p> <p>3、At most 10 target sequences can be detected in the same sample at the same time;</p> <p>4、The linear range is no less than 6 orders of magnitude;</p> <p>5、Good application expansibility, including for enrichment of NGs target sequence.</p> <p> </p> <p><strong>Applications：</strong></p> <p>1、Absolute quantification of  template;</p> <p>2、Detect rare mutation;</p> <p>3、CNV analysis;</p> <p>4、gene expression;</p> <p>5、WGA、target enrichment,etc.</p>

Analysis of digital single molecule gene expression system

Contact：Su, Xianbin
Tel：34204150

MORE <p><strong>Model： NanoString nCounter</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1、Fluorescent bar code probe labeling, single molecule detection and counting;</p> <p>2、No necessary for PCR amplification, high sample compatibility;</p> <p>3、Detect up to 800 aimed nucleic acids each time;</p> <p>4、Start with a minimun sample size of 100 ng, total RNA or 10000 cell lysates;</p> <p>5、Internal reference correlation R2 ≥ 0.95;</p> <p>6、The linear range is greater than 5 logarithmic thresholds;</p> <p>7、It takes 2.5 hours to prepare 12 samples and 2.7 hours to analyze 12 samples.</p> <p> </p> <p><strong>Applications：</strong></p> <p>1、Gene expression profile;</p> <p>2、miRNA expression analysis;</p> <p>3、Expression of  lncRNA;</p> <p>4、CNV analysis;</p> <p>5、Late validation of second generation sequencing</p>

Single-cell automatic preparation system

Contact：Su, Xianbin
Tel：34204150

MORE <p><strong>Model： Fluidigm C1</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1、Automatic single cell capture and nucleic acid sample preparation;</p> <p>2、A single chip can produce 96 or 800 single cell libraries at most;</p> <p>3、The initial cell number can be as low as 1000;</p> <p>4、It is suitable for cells with diameter of 5-10，10-17，17-25 μm;</p> <p>5、The cell capture and seperation efficiency is more than 90%(when 1000 cells in the sample)；</p> <p>6、The system has built-in bar code reader to automatically record the unique bar code of the chip;</p> <p>7、Semiconductor Peltier heating, temperature control range 4-99°C, heating speed > 4°C/s,  cooling speed > 3°C/s.</p> <p> </p> <p><strong>Applications：</strong></p> <p>1、prepare of single cell multi gene expression analysis library;</p> <p>2、prepare of single cell miRNA expression analysis library;</p> <p>3、prepare of single cell RNA sequencing library;</p> <p>4、prepare of single cell DNA sequencing library.</p>

Cytation 5 Cell Imaging Multi-Mode Reader

Contact：Hu, Youtian
Tel：34207545

MORE <p><strong>Model： Biotek Cytation 5</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1. Fluorescence system: the wavelength range of four grating system is 250-850 nm，and that of filter system is 200-850 nm, 9-50nm continuously adjustable，1nm step。Dynamic range: ≥7 orders of magnitudes;</p> <p>2. Chemiluminescence system: dynamic range: ≥6 orders of magnitude, with dynamic expansion function and dynamic expansion detection range. Light scanning can be carried out within the detection range, 1nm step，and the light scan diagram can be drawn. Detection mode: flash, glow, light scanning BRET, etc;</p> <p>3. Absorption light: wavelength range: 230-999 nm, 1 nm step. Bandwidth: 4nm (230-285nm)，8nm(>285nm)。Measurement range: 0-4.0 OD, resolution: 0.0001 OD;</p> <p>4. Well plate type: 6-1536 well plate、ultra micro multi volume detection plate. Temperature control: to 65℃，±0.2℃@ 37℃。 Plate reading speed：96 well plate≤11s，384 well plate ≤22s</p> <p> </p> <p><strong>Applications：</strong></p> <p>1. Quantification of DNA、RNA  and protein, purity analysis;</p> <p>2. Enzyme activity, enzyme kinetics detection;</p> <p>3. ELISAs and AlphaLISA;</p> <p>4. Cytotoxic analysis of MTS/LDH;</p> <p>5. AlphaScreen and FRET for the detection of  the interactions between molecules.</p>

Liquid automation workstation

Contact：Hu, Youtian
Tel：34207545

MORE <p><strong>Model：Beckman Coulter Biomek FXP</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1. Deck capacity: 24 deck positions meet the needs of high- throughput experiment;</p> <p>2. Transfer accuracy: 96-channel liquid transfer manipulator:  liquid transfer precision CV value 1μL : < 5%, 0.5μL < 10% Span-8 liquid transfer manipulator: liquid transfer precision CV value 1μL<5％，accuracy 1μL±3％</p> <p>3. Transfer range: 96-channel liquid transfer manipulator：low volume liquid transfer head 1-50μL，high volume liquid transfer head 5-200μL;     Span-8 liquid transfer manipulator: 0.5μL~5mL。</p> <p>4. Constant temperature incubation:  temerature range 0-100℃，temperature accuracy±0.3℃;</p> <p>5. High speed oscillation: vibration speed  0-1800rpm</p> <p>6. Openness: fully support the third-party suction head.</p> <p> </p> <p><strong>Applications：</strong></p> <p>1. Automatic application of drug screening. High-throughput screening, drug metabolism and kinetic analysis of leading compounds;</p> <p>2. Automatic application of genomics research. DNA microarray/sample preparation, extractions of DNA, plasmid and RNA, purifications of  PCR products and sequencing products, preparations of PCR reaction system, quantification and normalization of nucleic acids, replication of  bacteria or yeasts library.</p> <p>3. Automaic application of proteomics. Automatic protein crystallization screening workstation, in gel protein digestion, MALDI sampling, protein precipitation, protein interaction screening and peptide extraction in protein spectrum.</p>

High-performance liquid chromatography - triple-quadrupole mass spectrometry

Contact：Chen Ke
Tel：34208616

MORE <p><strong>Model： Agilent 6495 HPLC-QQQ-MS</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1. Flow rate range: 0.5-1.5 ml/min</p> <p>2. Pressure range: 1-15000 psi</p> <p>3. Column temperature range：5-65 ℃</p> <p>4. Mass to charge ratio（m/z）：5-1250 Da</p> <p>5. Resolution：0.7 amu</p> <p> </p> <p><strong>Applications：</strong></p> <p>1. Metabonomics: metabolite analysis of biological samples such as blood, urine, cell,etc.;</p> <p>2. Qualitative and quantitative analysis of  drug impurities;</p> <p>3. Clinical test analysis(drug and hormone test);</p> <p>4. Food safety detection and analysis(compound detection and analysis such as melamine, clenbuterol,etc.）</p> <p>5. Analysis of environmental pollutants(cosmetics and coatings, etc.)</p>

Behavioral analysis system of laboratory animals

Contact：Jia, Liling
Tel：34204467

MORE <p><strong>Model：PhenoScan Series Ⅱ</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1、3-point positioning, automatic behavior recognition function;    </p> <p>2、It can provide orbit recording and original  imaging display mode;    </p> <p>3、Automatic identification and  marking of footprint;    </p> <p>4、Corridor design, non forced or  induced way of laboratory animal ;    </p> <p>5、Multiple zones expansion function, up to 00 zones;    </p> <p>6、Support third-party of electrophysiological acquisition systems such as BIOPAC and DSI;    </p> <p>7、Support multiple video sources;    </p> <p>8、Support rotation test;    </p> <p>9、It can automatically determine three states: still, moving and exciting.</p> <p><strong>Applications：</strong></p> <p>Through the collection of video, photoelectricity and bioelectricity signals of animal behavior, combined with computer image processing, lattice analysis and bioelectricity signal analysis technology, the trace of animal behavior is extracted and indexes of animal behavior such as active behavior, learning and memory, conditioned reflex, reactivity and passive behavior are calculated.</p>

Leica Micromanipulator

Contact：Jia, Liling
Tel：34204467

MORE <p><strong>Model：Leica AM6000</strong></p> <p> </p> <p><strong>Applications：</strong></p> <p> 1. Observations of biological specimens and sections;    </p> <p>2. Embryo operation ( transgenic prokaryotic injection, somatic cell nuclear transfer);    </p> <p>3. Gene knockout and transgenic experiments.</p>

High content screening system

Contact：Hu, Youtian
Tel：34207545

MORE <p><strong>Model： MD ImageXpress Micro XLS</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1. Image quality (resolution and imaging area): 5.5 million sCMOS pixel camera;</p> <p>2. Imaging speed: >90 frames / sec;</p> <p>3. Filter: DAPI、CFP、 FITC/ALEXA488、 CY5、RHODAMINE/TRITC、TEXAS RED;</p> <p>4. Openness: support 6、12、24、48、96、384 、1536 well plate and culture dish.</p> <p> </p> <p><strong>Applications：</strong></p> <p>1. Screening of leading drugs;</p> <p>2. Cell cycle and apoptosis analysis;</p> <p>3. Multi wavelength cell count and high-throughput cell proliferation detection;</p> <p>4. High-throughput nuclear translocation analysis;</p> <p>5. Signal transduction and pathway screening.</p>

Liquid chromatography/quadrupole orbitrap mass spectrometry

Contact：Ku Xin
Tel：34204138

MORE <p><strong>Model：Q Exactive Plus</strong></p> <p> </p> <p><strong>Specifications：</strong></p> <p>1. Resolving power: 140,000; Scan rate: up to 12Hz; Dynamic range > 5000:1;</p> <p>2. Quadrupole mass filter; Multiple detection ability improve the efficiency of  single cycle analysis; S-Lens for better sensitivity.</p> <p> </p> <p><strong>Applications：</strong></p> <p style="margin: 0cm 0cm 0pt;">The Thermo Scientific™ Q Exactive™ Plus benchtop LC-MS/MS combines highperformance quadrupole precursor  selection with high-resolution, accuratemass (HR/AM) Orbitrap™ detection to deliver high performance and tremendous versatility. It takes Quanfirmation™ to a new level, making it possible to characterize, quantify, and confirm in a single analysis with a  single instrument. Technical enhancements to the  original Q Exactive instrument maximize  performance and reliability for large- and small-molecule applications. Advanced For Research Use Only. Not for use in diagnostic procedures.quadrupole technology optimizes precursor selection and transmission, improving quantitation of low-abundance ions in the most complex matrices. Sophisticated parallel reaction monitoring and dataindependent analysis provide reproducible quantitation results while delivering complete qualitative confidence. An optional intact protein mode enhances analysis of intact proteins through sophisticated ion beam control and easy collision pressure adjustment, while optional 280,000 maximum resolving power ensures  superior ID confidence in top-down and lipidomics applications.</p>

Living cell workstation

Contact：Man Zhang
Tel：34206535

MORE <p><strong>Model：</strong>Nikon Ti-E<br /> <br /> <strong>Specifications：</strong><br /> The living cell workstation based on research-oriented inverted fluorescence microscope is mainly used to automatically capture images of living cells changing with time. To study the various dynamic process of living cells, it is an important tool for the scientific research of cell biology, neurobiology, developmental biology and other disciplines. <br /> 1、It has the function of bright field, dark field, fluorescence and phase contrast, suitable for the observation of cell tissue, as well as the fluorescence observation and digital imaging of subcellular   structure and digital imaging;<br /> 2、Time-lapse sequence imaging, capture the images of cells changing with time, suitable for the observation of cell physiological and dynamic signals such as cell growth, metabolism, neurotransmission, signal transductions, etc.;<br /> 3、Multi-channel fluorescence imaging acquisition. For multi-target samples, the spatial relationship of different molecules and structures in cells is studied through obtaining multi-channel fluorescence image. <br /> 4、Multi dimensional image acquisition, automatic multi dimensional images acquisition, suitable for morphological spatial positioning research, spatial three-dimensional measurement, etc. ;<br /> 5、Perfect focus system, keep the focus from drifting when taking pictures for a long time ;<br /> 6、Internal reflection imaging functions to improve signal to noise ratio of fluorescence image.<br />  </p>

Laser scanning confocal microscope

Contact：Man Zhang
Tel：34206535

MORE <p><strong>Model：</strong>Nikon A1Si<br /> <br /> <strong>Specifications：</strong><br /> 1、Wavelength resolution: 2.5, 5,10 nm;  <br /> 2、Adopt 32 channel multi anode PMT;<br /> 3、Double integral signal processing;<br /> 4、Spectral detector with polarized light control technology;<br /> 5、Time dynamic recording of spectral image;<br /> 6、It can split the fluorescence signal spectrum and eliminate cross color;<br /> 7、High efficiency fluorescent transmission technology;<br /> 8、It can achieve a spectrum resolution of  up to 2.5nm;<br /> 9、True color samples can be observed  in real time;<br /> 10、Through transmission observation, the spectral detector can take both tramsmission and confocal images at the same time.<br /> <br /> <strong>Applications：</strong><br /> By using violet or visible light to excite fluorescence and processing computer images, we can not only observe the fixed cell, tissue slices, but also dynamically and real time observe and detect the living cells structure, molecule and ions.</p>

Flow cytometer(cell sorter)

Contact：Na Wang
Tel：34206124

MORE <p><strong>Model：</strong>Beckman  Moflo XDP<br /> <br /> <strong>Specifications：</strong><br /> 1、Sorting speed: ＞70,000 cells/sec<br /> 2、Sorting channel:4<br /> 3、Laser system: 355 nm，488 nm，635 nm<br /> 4、Sample tube adapter: 0.5 ml or 1.5 ml centrifuge tube, 12mm×75mm ordinary flow tube, 15 ml and 50 ml centrifuge tube;<br /> 5、Adapter of sorting cells collection tube: 0.5 ml and 1.5 ml centrifuge tubes, 12mm×75mm  ordinary flow tube, 6-1536 orifice plate, glass slide<br /> 6、Optimal detection and sorting cell size range:0.2-30 µm;<br /> 7、Cell information obtained and stored in a single time:  1 billion cells<br /> 8、Available fluorescein labeling : FITC/GFP/CY3/YFP/PE/ECD/PI/PE-CY5/PerCP/TC/PE-CY7/APC/APC-   <br />       CY7/HOECHST33342/DAPI/FLUO-2/FLUO-3/Alexa Fluo;<br /> 9、Linear range of fluorescence: 5 logarithmic thresholds;<br /> 10、Nozzle types: 8 kinds, 50µm - 200µm<br /> <br /> <strong>Applications：</strong><br /> MoFlo XDP superfluid cell sorting system can  identify, classify, quantify and separate cells in complex samples. One or four specific cells can be simultaneously purified by superfluid sorting, high-throughput monoclonal sorting or cell chip preparation at a single time. These selected cells can be directly used for culture, transplantation, nucleic acid extraction, single cell PCR amplification or in situ hybridization, etc., which can further study the gene, protein, functional level of cells and the differences between different cells. The percentage of discarded cells in hardware is less than 5%, which ensures the high recycle rate of target cells in the sample, especially suitable for cell research with extremely low content such as stem cells. MoFlo XDP superfluid cell sorting system can be used to detect and sort all kinds of eukaryotic and prokaryotic cells, plant cells, microorganisms, plankton, etc.</p>

Flow cytometer

Contact：Na Wang
Tel：34206124

MORE <p><strong>Model：</strong>LSR Fortessa<br /> <br /> <strong>Specifications：</strong><br />  1、Laser system: 355 nm，405 nm，488 nm，640 nm<br />  2、Data acquisition speed: 40,000 events/second <br />  3、Sample flow rate: low speed 12µl/min，medium speed 35µl/min，high speed 60µl/min<br />  4、Fluorescence sensitivity: FITC 80 MESF，PE 30 MESF，PE-CY5 10 MESF，APC 70 MESF<br />  5、Fluorescence resolution: CV<3%<br />  6、Fluorescence linearity：the ratio of Doublet/Singlet is 1.95-2.05<br /> <br /> <strong>Applications：</strong><br /> Rapid analysis of cell suspension was carried out. By detecting the cells arranged in a single row in the flowing liquid one by one, the light scattering and fluorescence indexes of the cells were obtained, and the physical and chemical characteristics of the internal structure, DNA, protein, etc. were analyzed, mainly used for cell cycle analysis, cell apoptosis analysis, cell surface or internal markers and cell physiology research.<br />  </p>

The NextSeq 500 system

Contact：Xianbin Su
Tel：34204150

MORE <p><strong>Model：</strong>illumina NextSeq 500<br /> <br /> <strong>Specifications：</strong><br /> 1、 Flow cells are available in configurations for high output and mid output .(400M or 130M read count/output);<br /> 2、High output support 1×75，2×75，2×150 bp read length；mid output support 2×75，2×150 bp read length;<br /> 3、Quality score: Q30>75%;<br /> 4、It can generate 120 GB of data in 29 hours at most; <br /> 5、It can only run one library at a time.<br /> <br /> <strong>Applications：</strong><br /> 1、Complete transcriptome sequencing;<br /> 2、Whole exome sequencing;<br /> 3、Target sequencing; <br /> 4、De novo sequencing; <br /> 5、Single cell sequencing<br />  </p>