课题组简介：以22项授权发明专利为基础，开发了可用于高通量DNA测序的系列荧光标记核苷酸。这些可逆终止核苷酸(reversible terminators)可以实现100%单碱基延伸后的有效终止以及100%断裂后的持续延伸，该类可逆终止核苷酸可同时用于二代合成测序，也可以用于三代单分子合成测序。当模板存在连续多个相同碱基时，仍然可实现一次测序循环只延伸一个可逆终止核苷酸。

1. Lianjiang Tan, Yazhi Liu, Qinglai Yang, Xiaowei Li, Xin-Yan Wu, Bing Gong, Yu-Mei Shen*, Zhifeng Shao
Design and synthesis of fluorescence-labelled nucleotide with azo cleavable linkers for DNA sequencing
Chem. Commun., 2016, 52, 954-957.

2. Lianjiang Tan, Yazhi Liu, Xiaowei Li, Xin-Yan Wu, Bing Gong, Yu-Mei Shen*, Zhifeng Shao
Design and synthesis of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing
Chem. Commun., 2016, 52, 2549-2552.

3. Pingyang, Yumei Shen
Rapidly photocleavable triazene-functionalized nucleotides for DNA sequencing
Org. Biomol. Chem., 2026, 24, 4835.

4. Bo-Wei Tang, Ping-Yang Wang and Yu-Mei Shen*
Design and synthesis of fluorescent-dye-labeled nucleotide with a new cleavable azo linker for DNA sequencing by synthesis
Org. Biomol. Chem., 2025, 23, 2456

5. Pingyang, Bowei Tang, Yumei Shen
Synthesis and Evaluation of Cleavable Thiazolidine-Modiffed Nucleotides for DNA Sequencing
Journal of Organic Chemistry 2025, 90,9622.